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DOI: 10.1055/s-0037-1613836
Cloning of Guinea Pig Tissue Factor cDNA: Comparison of Primary Structure among Six Mammalian Species
This work was supported in part by grant HL30616, HL07152, and AI40689 from the National Institutes of Health, Bethesda, Maryland.
Publication History
Received
03 August 1999
Accepted after revision
05 November 1999
Publication Date:
14 December 2017 (online)
Summary
Tissue factor (TF) is a transmembrane glycoprotein that serves as an essential cofactor for plasma coagulation factor VII. TF procoagulant activity exhibits varying species specificity. In particular, guinea pig (GP) TF is unable to activate clotting in heterologous plasma systems, but the molecular basis for this phenomenon is not yet understood. The full-length GP TF cDNA was cloned and sequenced. The open reading frame encoded a predicted precursor protein of 289 amino acids (aa) which was expressed in a reticulocyte lysate system as a protein of apparent molecular weight of 34 kD. The identity of the predicted aa sequence of mature GP TF with rabbit, human, bovine, rat and mouse TF was 66.4, 64.4, 60.6, 53.2 and 52.2%, respectively. With a focus on sites of potential functional significance, we compared sequences within the known binding regions. The eleven residues at the interface region between the TF1 and TF2 modules, which bind to the EGF domain of VIIa, were perfectly conserved among the six species, with the exception of an isoleucine replacing a lysine in the guinea pig sequence. However, only four of the eleven binding residues in the TF1 module, known to interact with the catalytic domain of factor VII, and three of the five residues in the TF2 module, involved in binding the factor VII Gla domain, were conserved among species. We hypothesize that divergence at these regions contributes to the specificity and non-reciprocity of TF procoagulant activity between species.
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References
- 1 Bach RR. Initiation of coagulation by tissue factor. CRC Crit Rev Biochem 1988; 23 (04) 339-68.
- 2 Edgington TS, Mackman N, Brand K, Ruf W. The structural biology of expression and function of tissue factor. Thromb Haemost 1991; 66 (01) 67-79.
- 3 Radcliffe R, Nemerson Y. Activation and control of factor VII by activated factor X and thrombin. Isolation and characterization of a single chain form of factor VII. J Biol Chem 1975; 250 (02) 388-95.
- 4 Osterud B, Rapaport SI. Activation of factor IX by the reaction product of tissue factor and factor VII: additional pathway for initiating blood coagulation. Proceedings of the National Academy of Sciences of the United States of America 1977; 74 (12) 5260-4.
- 5 Muller YA, Ultsch MH, Kelley RF, de Vos AM. Structure of the extracellular domain of human tissue factor: location of the factor VIIa binding site. Biochemistry 1994; 33 (36) 10864-70.
- 6 Harlos K, Martin DM, O’Brien DP, Jones EY, Stuart DI, Polikarpov I. et al. Crystal structure of the extracellular region of human tissue factor [published erratum appears in Nature 1994 Oct 20; 371 (6499): 720]. Nature 1994; 370 6491 662-6.
- 7 Schullek JR, Ruf W, Edgington TS. Key ligand interface residues in tissue factor contribute independently to factor VIIa binding. J Biol Chem 1994; 269 (30) 19399-403.
- 8 Ruf W, Schullek JR, Stone MJ, Edgington TS. Mutational mapping of functional residues in tissue factor: identification of factor VII recognition determinants in both structural modules of the predicted cytokine receptor homology domain. Biochemistry 1994; 33 (06) 1565-72.
- 9 Gibbs CS, McCurdy SN, Leung LL, Paborsky LR. Identification of the factor VIIa binding site on tissue factor by homologous loop swap and alanine scanning mutagenesis. Biochemistry 1994; 33 (47) 14003-10.
- 10 Ruf W, Kelly CR, Schullek JR, Martin DM, Polikarpov I, Boys CW. et al. Energetic contributions and topographical organization of ligand binding residues of tissue factor. Biochemistry 1995; 34 (19) 6310-5.
- 11 Kelley RF, Costas KE, O’Connell MP, Lazarus RA. Analysis of the factor VIIa binding site on human tissue factor: effects of tissue factor mutations on the kinetics and thermodynamics of binding. Biochemistry 1995; 34 (33) 10383-92.
- 12 Banner DW, D’Arcy A, Chene C, Winkler FK, Guha A, Konigsberg WH. et al. The crystal structure of the complex of blood coagulation factor VIIa with soluble tissue factor [see comments]. Nature 1996; 380 6569 41-6.
- 13 Lyberg T, Hetland O, Prydz H. Synthesis of thromboplastin protein by a murine macrophage-like cell line. Thromb Haemost 1982; 47 (02) 154-6.
- 14 Kadish JL, Wenc KM, Dvorak HF. Tissue factor activity of normal and neoplastic cells: quantitation and species specificity. J Nat Cancer Inst 1983; 70 (03) 551-7.
- 15 Janson TL, Stormorken H, Prydz H. Species specificity of tissue thromboplastin. Haemostasis 1984; 14 (05) 440-4.
- 16 Fang CH, Lin TC, Guha A, Nemerson Y, Konigsberg WH. Activation of factor X by factor VIIa complexed with human-mouse tissue factor chimeras requires human exon 3. Thromb Haemost 1996; 76 (03) 361-8.
- 17 Andrews BS, Rehemtulla A, Fowler BJ, Edgington TS, Mackman N. Conservation of tissue factor primary sequence among three mammalian species. Gene 1991; 98 (02) 265-9.
- 18 Scarpati EM, Wen D, Broze Jr GJ, Miletich JP, Flandermeyer RR, Siegel NR. et al. Human tissue factor: cDNA sequence and chromosome localization of the gene. Biochemistry 1987; 26 (17) 5234-8.
- 19 Takayenoki Y, Muta T, Miyata T, Iwanaga S. cDNA and amino acid sequences of bovine tissue factor. Biochem Biophys Res Com 1991; 181 (03) 1145-50.
- 20 Taby O, Rosenfield CL, Bogdanov V, Nemerson Y, Taubman MB. Cloning of the rat tissue factor cDNA and promoter: identification of a serum-response region. Thromb Haemost 1996; 76 (05) 697-702.
- 21 Ranganathan G, Blatti SP, Subramaniam M, Fass DN, Maihle NJ, Getz MJ. Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type beta 1. J Biol Chem 1991; 266 (01) 496-501.
- 22 Russo P, Li WZ, Hampsey DM, Zaret KS, Sherman F. Distinct cis-acting signals enhance 3’ endpoint formation of CYC1 mRNA in the yeast Saccharomyces cerevisiae. EMBO Journal 1991; 10 (03) 563-71.
- 23 Shi RJ, Simpson-Haidaris PJ, Lerner NB, Marder VJ, Silverman DJ, Sporn LA. Transcriptional regulation of endothelial cell tissue factor expression during Rickettsia rickettsii infection: involvement of the transcription factor NF-kappaB. Infection & Immunity 1998; 66 (03) 1070-5.
- 24 Schaefer BC. Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends. Anal Biochem 1995; 227 (02) 255-73.
- 25 Proudfoot N. The end of the message [news]. Nature 1982; 298 5874 516-7.
- 26 Proudfoot N. The end of the message and beyond [news]. Nature 1984; 307 5950 412-3.
- 27 Caput D, Beutler B, Hartog K, Thayer R, Brown-Shimer S, Cerami A. Identification of a common nucleotide sequence in the 3’-untranslated region of mRNA molecules specifying inflammatory mediators. Proceedings of the National Academy of Sciences of the United States of America 1986; 83 (06) 1670-4.
- 28 Shaw G, Kamen R. A conserved AU sequence from the 3’ untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 1986; 46 (05) 659-67.
- 29 von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Europ J Biochem 1983; 133 (01) 17-21.
- 30 von Heijne G. Signal sequences. The limits of variation. J Mol Biol 1985; 184 (01) 99-105.
- 31 Morrissey JH, Fakhrai H, Edgington TS. Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade. Cell 1987; 50 (01) 129-35.
- 32 Edgington TS, Dickinson CD, Ruf W. The structural basis of function of the TF. VIIa complex in the cellular initiation of coagulation. Thromb Haemost 1997; 78 (01) 401-5.
- 33 Kelly CR, Schullek JR, Ruf W, Edgington TS. Tissue Factor residue Asp44 regulates catalytic function of the bound proteinase Factor VIIa. Biochem J 1996; 315 Pt1 145-51.